苯丙氨酸解氨酶 (Phenylalanineammonialyase ,PALE .C .4.3 .1.5 )是一种只存在于植物及微生物细胞内的酶 ,主要分布于高等植物如小麦、大豆、玉米、土豆及酵母[1] 、真菌[2 ] 、链霉菌[3]中 .在 pH 8.8时 ,PAL催化苯丙氨酸脱氨生成肉桂酸和氨 ,当过量的氨存在 ,如 pH 10时 ,PAL催化肉桂酸和氨 ,氨化加成生成L 苯丙氨酸 (L phenylalanine) .利用PAL这种逆向催化特性转化肉桂酸生产L Phe ,已在国内外投入商业规模开发 ,成为当前生物化工领域研究与开发的热点 .L Phe是人…
L-phenylalanine is an important amino acid in commercial production of food industry and medicine nowaday. The biotransformations of trans-cinnamic acid to L-phenylalanine using phenylalanine ammonia- lyase(PAL) is in the front of research and development now.This article reviews the research progress in molecular biology of Phenylalanine ammonia-lyase (PAL) as an important enzyme for production of L-phenylalanine. Ref 43